WebSeveral methods for the detection of phospholipids have been reported (29-37). They are summarized in Table 1. In-vitro screening assays using the fluorescently tagged lipid NBD-PE, the fluorescent probe Nile red as well as the phosphatidylcholine-conjugated dye NBD-PC were previously used to determine intracellular phospholipids (29, 30, 34). WebPhospholipidosis (PL) is lysosomal storage disorder characterized by the excessive accumulation of intracellular phospholipids (Figure 1). The mechanism of …

HCS LipidTOX™ Red Neutral Lipid Stain, for cellular imaging

WebDec 7, 2024 · Annexin V-FITC/PI apoptosis detection kit was acquired from BD (Franklin Lakes, NJ, USA). Then, Hoechst 33342, eBioscience™ JC-1 Mitochondrial Membrane Potential Dye, Cell ROX Deep Red, and HCS LipidTOX™ Green Phospholipidosis Detection Reagent were obtained from Thermo Fisher Scientific (Waltham, MA, USA). WebJan 27, 2024 · Briefly, cells were incubated in complete bronchial epithelial growth medium supplemented with 1 x phospholipidosis detection reagent for 48 hr. Cells were subsequently fixed in 4% PFA and stained with 1 X neutral lipid detected reagent for 30 min and counterstained with 1 μg mL-1 Hoechst 33258. Staining intensity was normalized to … how do loc interest rates work

Different Sensitivity of Macrophages to Phospholipidosis …

WebSep 3, 2012 · Drug-induced phospholipidosis (PLD) is a lysosomal storage disorder characterized by the accumulation of phospholipids within the lysosome. ... After 48 h, the medium was replaced with fresh medium that included the test substances and HCS LipidTOX Green phospholipidosis detection reagent (Invitrogen, San Diego, US) at their … WebMar 1, 2024 · LipidTOX Green Phospholipidosis Detection Reagent (LipidTOX, Invitrogen, Carlsbad, CA, USA) was applied to confirm induction of phospholipidosis by selected drugs. According to the manufacturer's protocol, LipidTOX was diluted in full media (1:500) and then filtered with a syringe filter (2 μm in pores size). ... Webreagents containing DMSO using equipment and practices appropriate for the hazards posed by such materials. Dispose of the reagents in compliance with all pertaining local … how much potatoes per person kg