Crystallin promoter
WebThis study concluded that there existed a TGF-β responsive element in the promoter just 285 base pairs upstream of the transcriptional start site ... porcine TGF-β1 under the lens-specific αA-crystallin promoter and then another similar line was created but with the gene driven by the βb-crystallin promoter, corresponding to another lens ... WebDec 3, 2013 · CpG island methylation in the αA-crystallin promoter was similar in age-related cataract and high-myopic cataract classified as Lens Opacity Classification System III Grade Nuclear Color 2–3. Pyrosequencing, a real-time DNA sequencing method, was used to determine single-base variations caused by CpG methylation of the CRYAA …
Crystallin promoter
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WebDec 29, 2008 · The scallop Ω-crystallin promoter sequence contains an abundance of motifs that are similar to those in vertebrate crystallin promoters. These include cis … WebThis present study is the first to show that mammalian α-crystallin promoter function can be analyzed in zebrafish embryos by observing green fluorescent protein (GFP) …
WebJul 1, 2002 · It is proposed that Pax6 occupies and represses the chicken βB1-crystallin promoter in lens epithelial cells, and is displaced by Prox1 and Mafs, which activate the promoter, in differentiating cortical fiber cells. ... It is shown that the transactivation potential of c-Maf and MafB for the rat gammaD-crystallin Maf-responsive element (gammaD ... WebMar 1, 1989 · Recently Landel et al. (1988) have combined the α -crystallin promoter with the ricin cytotoxic gene to produce a single line of transgenic mice with a somewhat more severe phenotype consisting of a still smaller lens and other interesting eye malformations.
WebJul 5, 2002 · Crystallin promoter reporters. The constructs with the αA-, βB2-, and γF-crystallin promoters linked to a luciferase reporter are depicted. Potential c-Maf recognition sites are located at −110 to −98 in the αA and βB2 promoters, and at −47 to −35 in the γF promoter ( 30, 31, 32 ). WebBy use of a gene fusion of the alpha A-crystallin promoter to the viral immortalizing oncogene, polyoma large T antigen (PyLT), we created transgenic mice that express PyLT specifically in ocular lens. Expression of large T antigen during embryonic development led to a perturbation in lens development, specifically, an interference with the ...
WebNov 3, 2003 · In order to identify PaxB response elements in the J3-crystallin promoter, we generated a series of J3 promoter deletions in the luciferase reporter construct. The shortest promoter fragment, consisting of only 200 bp upstream of the transcriptional start site, was activated by PaxB and not by PaxB(I84R) (data not shown; but cf. Figure 5C ) .
WebSep 1, 2002 · Thus, the chicken betaB1-crystallin promoter directs high level RNA expression to lens fiber cells, which may be especially useful for the expression of ribozyme and anti-sense RNAs in addition to ... can acid in stomach cause chest painWebThe human papillomavirus type 16 (HPV-16) E6 and E7 oncogenes are thought to play a role in the development of most human cervical cancers. These E6 and E7 oncoproteins affect cell growth control at least in part through their association with and can acid make deoression worse after tripWebFeb 21, 2024 · The predicted promoter probability and location, and the type of the mutation are shown above each sequence (TSS at position 100). b–d, TERT promoter point mutations predicted by the CNN model... fish conveyor beltWebSox-1 transactivated the γF-crystallin promoter in a dose-dependent fashion, similar to c-Maf (Fig. 2 C). Sox-1 also stimulated the βB2 promoter, but less strongly than c-Maf (Fig. 2 B). Prox-1 (80 ng) stimulated the βB2- and γF-crystallin promoters only about 2-fold (Fig. 2, B and C). To determine whether these transcription factors can ... can acidity be curedWebCrystallins are a diverse group of abundant soluble proteins that are responsible for the refractive properties of the transparent eye lens. We showed previously that Pax-6 can activate the αB-crystallin/small heat shock protein promoter via the lens-specific regulatory regions LSR1 (−147/−118) and LSR2 (−78/−46). fish cookbookWebgene-specific sequences such as the gene-specific promoter sequence in the proximal promoter of the small heat shock protein gene, alpha B-crystallin (cryab) , may have critical roles in regulating gene-specific activity from eukaryotic promoters. AlphaB-crystallin is a novel regulator of brain metastasis in triple-negative breast cancer. fish conway arWebApr 8, 2024 · Further explorations unveiled the increased MAF in high myopia and its participation in a direct activation of crystallin promoters and an indirect activation via … can acidosis cause heart block